To obtain a copy of the report

Validation Report Includes:
Human IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6 IL-8, IL-10, IL-12p70, TNFα (Active Trimer)

The Ciraplex® immunoassay is a multiplex sandwich ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative measurement of target proteins in serum, EDTA plasma, and tissue culture supernatants. Each well of the 96-well microplate is pre-spotted with protein-specific antibodies and these antibodies capture specific proteins from the standards and samples of interest. After unbound proteins are washed away, biotinylated detecting antibodies are added and bind to a secondary site on the target proteins. After the removal of excess detection antibody, streptavidin-horseradish peroxidase (SA-HRP) is added. HRP is an enzyme that reacts with a substrate to produce a luminescent signal that is detected by the Cirascan™ Imaging System. The intensity of the signal produced is directly proportional to the quantity of each protein in the standard or sample of interest.
Click here for a menu of our Ciraplex® Assays

Aushon BioSystems
43 Manning Rd
Billerica, MA 01821